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Environmental Culture
MessageThis procedure was implemented to ensure that current sterilization is adequate to prevent contamination. The Kilit Ampule is used as a biological indicator for saturated steam sterilization cycles.
Test Code
Lab1020
CPT Codes
87081
Preferred Specimen
ESwab – device
Kilit ampule – autoclave monitoring
Kilit ampule – autoclave monitoring
Instructions
Devices:
1. Swab appropriate area of device using swab in ESwab container.
Note: For dry areas to be sampled, moisten swab with sterile water.
2. Send samples to the lab in a timely manner.
1. Swab appropriate area of device using swab in ESwab container.
Note: For dry areas to be sampled, moisten swab with sterile water.
2. Send samples to the lab in a timely manner.
Autoclaves:
1. Place an adequate number of ampules in load and run autoclave on a sterilization cycle.
2. Send ampule to the lab in a timely manner.
1. Place an adequate number of ampules in load and run autoclave on a sterilization cycle.
2. Send ampule to the lab in a timely manner.
Transport Container
ESwab
Kilit Ampule
Kilit Ampule
Transport Temperature
Ambient
Specimen Stability
Ambient – 24 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Improper labeling
Improper transport of specimen
Improper transport of specimen
Methodology
Device: Conventional culture techniques
Kilit: Mesa Labs Biological indicator incubator
Setup Schedule
Daily
Reference Range
No growth
KILIT Result: Negative
KILIT Result: Negative
Clinical Significance
Confirmation that sterilization techniques are adequate to prevent contamination.
Performing Laboratory
Meritus Microbiology
