Vitamin B1 (Thiamine), Blood, LCMSMS

Test Code
VITB1

CPT Codes
84425

Preferred Specimen
3 mL whole blood collected in an EDTA (lavender-top) tube - protect from light

Minimum Volume
1.1 mL

Other Acceptable Specimens
Whole blood collected in: Sodium heparin (green-top) tube • Lithium heparin (green-top) tube

Instructions
Transfer whole blood to a plastic shipping vial to prevent breakage. Wrap tube in aluminum foil to protect from light. Freeze immediately.



Note: Samples received frozen, but not protected from light are acceptable. Wrap sample in foil before sending to lab. No disclaimer is required. Samples received refrigerated but not protected from light are unacceptable.

Transport Container
Plastic screw-cap vial protected from light

Transport Temperature
Frozen immediately

Specimen Stability
Room temperature: 4 hours

Refrigerated: 24 hours

Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received thawed • Received room temperature • Received refrigerated

Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Reference Range
Adults: 78-185 nmol/L

Clinical Significance
Vitamin B1 is required for branched-chain amino acid and carbohydrate metabolism. Vitamin B1 deficiency is most often due to alcoholism or chronic illness. In the early stage, patients with vitamin B1 deficiency exhibit anorexia, irritability, apathy, and generalized weakness. Prolonged deficiency causes beriberi.

Performed By
Quest Diagnostics Nichols Institute



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.