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Vitamin B1 (Thiamine), Blood, LCMSMSTest Code
3 mL whole blood collected in an EDTA (lavender-top) tube - protect from light
Other Acceptable Specimens
Whole blood collected in: Sodium heparin (green-top) tube • Lithium heparin (green-top) tube
Transfer whole blood to a plastic shipping vial to prevent breakage. Wrap tube in aluminum foil to protect from light. Freeze immediately.
Note: Samples received frozen, but not protected from light are acceptable. Wrap sample in foil before sending to lab. No disclaimer is required. Samples received refrigerated but not protected from light are unacceptable.
Plastic screw-cap vial protected from light
Room temperature: 4 hours
Refrigerated: 24 hours
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received thawed • Received room temperature • Received refrigerated
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Adults: 78-185 nmol/L
Vitamin B1 is required for branched-chain amino acid and carbohydrate metabolism. Vitamin B1 deficiency is most often due to alcoholism or chronic illness. In the early stage, patients with vitamin B1 deficiency exhibit anorexia, irritability, apathy, and generalized weakness. Prolonged deficiency causes beriberi.
Quest Diagnostics Nichols Institute