|Test List||A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #|
Vitamin B6 (Pyridoxine), PlasmaTest Code
1 mL frozen plasma, light protected, collected in an EDTA (lavender-top) tube
Draw blood into light-protected lavender-top evacuated tube, following an overnight fast.
Separation of cells can't be performed immediately after collection, keep the whole blood refrigerated and protected from light. The separation of cells must be completed within 6 hours. Separate cells by centrifugation at 2-8ºC (2200-2500 rpm, 800-1000 x g) for 5-10 minutes. Transfer plasma to dark brown polypropelene or polyethylene transport tubes to protect from light. Alternately, neutral color polypropylene or polyethylene tubes can be used if wrapped in aluminum foil. Freeze the tubes -10ºC to -30ºC. Ship frozen.
Plastic screw-cap vial
Room temperature: 6 hours
Refrigerated: 12 hours
Frozen -20°C: 6 days
Frozen -70°C: 42 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens not protected from light • Received refrigerated • Received room temperatures • Collected in tubes other than lavender-top tubes • Lipemia • Hemolysis • Improperly labeled tubes
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Set up: Mon-Fri (night shift); Report available: 4 days
|2-17 Years||3.0-35.0 ng/mL|
Vitamin B6 is a cofactor in many metabolic pathways including heme synthesis. Vitamin B6 deficiency may be observed in patients with metabolic disorders, secondary to therapeutic drug use, or alcoholism. Deficiency affects the function of the immune system.
Quest Diagnostics Nichols Institute