Vitamin B6 (Pyridoxine), Plasma

Test Code
VB6

CPT Codes
84207

Preferred Specimen
1 mL frozen plasma, light protected, collected in an EDTA (lavender-top) tube

Minimum Volume
0.5 mL

Instructions
Draw blood into light-protected lavender-top evacuated tube, following an overnight fast.

Separation of cells can't be performed immediately after collection, keep the whole blood refrigerated and protected from light. The separation of cells must be completed within 6 hours. Separate cells by centrifugation at 2-8ºC (2200-2500 rpm, 800-1000 x g) for 5-10 minutes. Transfer plasma to dark brown polypropelene or polyethylene transport tubes to protect from light. Alternately, neutral color polypropylene or polyethylene tubes can be used if wrapped in aluminum foil. Freeze the tubes -10ºC to -30ºC. Ship frozen.

Transport Container
Plastic screw-cap vial

Transport Temperature
Frozen

Specimen Stability

Room temperature: 6 hours
Refrigerated: 12 hours
Frozen -20°C: 6 days
Frozen -70°C: 42 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens not protected from light •  Received refrigerated • Received room temperatures • Collected in tubes other than lavender-top tubes • Lipemia • Hemolysis • Improperly labeled tubes

Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Setup Schedule
Set up: Mon-Fri (night shift); Report available: 4 days

Reference Range
2-17 Years 3.0-35.0 ng/mL
Adult 2.1-21.7 ng/mL


Clinical Significance
Vitamin B6 is a cofactor in many metabolic pathways including heme synthesis. Vitamin B6 deficiency may be observed in patients with metabolic disorders, secondary to therapeutic drug use, or alcoholism. Deficiency affects the function of the immune system.

Performed By
Quest Diagnostics Nichols Institute



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.