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Varicella-Zoster Virus (VZV) Real-Time Quantitative PCRTest Code
1 mL whole blood collected in an EDTA (lavender-top) tube, or 1 mL CSF, or Bronchial brush/wash submitted in a sterile leak-proof container, or 1 mL swab submitted in VCM or M4 Transport Medium
Other Acceptable Specimens
1 mL whole blood collected in: ACD-B (yellow-top) tube, or EDTA (dark blue-top) tube • Throat swab submitted in VCM or M4 Transport Medium • 1 mL eye fluid submitted in sterile leak-proof container
Whole blood must be shipped refrigerated (cold packs). Frozen whole blood is not acceptable for this assay.
Swabs in M4 or VCM, sterile or dry, are acceptable refrigerated for 7 days or frozen for 30 days.
Swabs (dry, in M4 or VCM Medium) are acceptable to include eye swab, nasopharyngeal swab, throat swab, and lesion (vesicle) aspirate/swab).
Sterile screw-cap container
Refrigerated (cold packs)
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days (do not freeze whole blood)
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Frozen whole blood
Real-Time Polymerase Chain Reaction (RT-PCR)
This test was developed and its performance characteristics have been determined by Quest Diagnostics. Performance characteristics refer to the analytical performance of the test.
This test is performed pursuant to a license agreement with Roche Molecular Systems, Inc.
Varicella-Zoster virus (VZV) is a member of the Herpesviridae family that causes chickenpox as a primary infection and can reactivate later in life as herpes zoster or shingles. VZV infection in immunocompromised individuals often leads to a progressive disease state involving multiple organs. The detection of Varicella-Zoster Virus (VZV) DNA is based upon the real-time amplification and detection of specific VZV genomic DNA sequences by PCR from total DNA extracted from the specimen. The quantitative range of this assay is 500-2,000,000 copies/mL.
Quest Diagnostics Nichols Institute