Cortisol Stimulation Test, Plasma or Serum

Test Code
7383


CPT Codes
80400-Adrenocorticotropic hormone stimulation panel; 82533-Cortisol, total

Preferred Specimen
Submit only 1 of the following specimens:

Plasma
Container/Tube: Green-top (heparin) tube(s)
Specimen Volume: 3 mL (minimum volume: 1 mL) of plasma for each specimen
Collection Instructions: 1. Draw a baseline cortisol at least 30-minutes after insertion of stopcock. 2. Draw cortisol 30- and 60-minutes after injection of Cortrosyn. 3. Separate plasma from red cells within 1 hour.
Note: 1. Do not begin test immediately after Cortrosyn administration. 2. Draw all specimens from stopcock. 3. Indicate plasma and as 0-, 30-, or 60-minutes. 4. Label specimen appropriately (plasma and as 0-, 30-, or 60-minutes).

Serum
Container/Tube: Serum gel tube(s)
Specimen Volume: 3 mL (minimum volume: 1 mL) of serum for each specimen
Collection Instructions: 1. Draw a baseline cortisol at least 30-minutes after insertion of stopcock. 2. Draw cortisol 30- and 60-minutes after injection of Cortrosyn. 3. Separate serum from red cells within 1 hour.
Note: 1. Do not begin test immediately after Cortrosyn administration. 2. Draw all specimens from stopcock. 3. Indicate serum and as 0-, 30-, or 60-minutes. 4. Label specimen appropriately (serum and as 0-, 30-, or 60-minutes). "


Minimum Volume
3 ml (minimum volume: 1 ml) of plasma for each specimen


Transport Temperature
Refrigerate


Methodology
Immunochemiluminescent Assay

Setup Schedule
Monday through Sunday


Reference Range
Normal response is a rise in serum cortisol >=7 µg/dL and peak response >20 µg/dL


Clinical Significance
Useful as an adrenal function test, for diagnosing Addison’s disease, and primary adrenal hypofunction. It is also used as a means of differentiating between hypopituitarism versus hypoadrenalism  


Performed By
CoxHealth



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.