Complement, Total (CH50)

Test Code
618

CPT Codes
86162

Preferred Specimen
1 mL serum 

Minimum Volume
0.2 mL

Instructions
Avoid hemolysis. Centrifuge serum specimens within 1 hour of collection. Immediately pipette serum into sterile, plastic, screw-capped vial(s) and freeze solid -20°C or lower. Do not allow samples to thaw. With multiple tests, submit a separate tube for each test. Send frozen samples on dry ice to the laboratory.

Transport Container
Plastic screw-cap vial

Transport Temperature
Frozen

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
CSF, Pleural Fluid, Pericardial Fluid, Synovial Fluid

Methodology
Liposome

Setup Schedule
Set up: Mon-Sat a.m.; Report available: Next day

Reference Range
31-60 U/mL

Clinical Significance
CH50 is a screening test for total complement activity. Levels of complement may be depressed in genetic deficiency, liver disease, chronic glomerulonephritis, rheumatoid arthritis, hemolytic anemias, graft rejection, systemic lupus erythematosis, acute glomerulonephritis, subacute bacterial endocarditis and cryoglobulinemia. Elevated complement may be found in acute inflammatory conditions, leukemia, Hodgkin's Disease, sarcoma, and Behcet's Disease.

Performed By
Quest Diagnostics Nichols Institute



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.