ANA Screen, IFA with Reflex to Titer and Pattern, IFA

Test Code
249

CPT Codes
86038

Includes
If ANA Screen, IFA is positive, ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).

Preferred Specimen

1 mL Serum collected in Separator tube (SST® - plastic only)

Minimum Volume
0.5 mL

Other Acceptable Specimens

1 mL Serum collected in a Red-top tube (no gel)

Instructions

Samples that have gross hemolysis, lipemia, or icterus are unacceptable; however, transport at room temperature is acceptable.

Transport Container
Plastic screw-cap vial

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 4 days
Refrigerated: 7 days
Frozen: 30 days

Methodology
Immunofluorescence Assay (IFA)

Reference Range

ANA Screen	Negative
ANA Titer
<1:40	Negative
1:40-1:80	Low antibody level
>1:80	Elevated antibody level

Clinical Significance
Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE.

Performed By
Quest Diagnostics Nichols Institute