ANA Screen, IFA with Reflex to Titer and Pattern, IFA

Test Code

CPT Codes

If ANA Screen, IFA is positive, ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).

Preferred Specimen
1 mL Serum collected in Separator tube (SST® - plastic only)

Minimum Volume
0.5 mL

Other Acceptable Specimens
1 mL Serum collected in a Red-top tube (no gel)

Samples that have gross hemolysis, lipemia, or icterus are unacceptable; however, transport at room temperature is acceptable. 

Transport Container
Plastic screw-cap vial

Transport Temperature
Refrigerated (cold packs)

Specimen Stability

Room temperature: 4 days

Refrigerated: 7 days

Frozen: 30 days

Immunofluorescence Assay (IFA)

Reference Range
ANA Screen Negative
ANA Titer  
<1:40 Negative
1:40-1:80 Low antibody level
>1:80 Elevated antibody level

Clinical Significance
Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.