ANA Screen, IFA with Reflex to Titer and Pattern, IFA

Test Code
249

CPT Codes
86038

Includes
If ANA Screen, IFA is positive, ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).

Preferred Specimen
1 mL Serum collected in Separator tube (SST® - plastic only)


Minimum Volume
0.5 mL

Other Acceptable Specimens
1 mL Serum collected in a Red-top tube (no gel)


Instructions
Samples that have gross hemolysis, lipemia, or icterus are unacceptable; however, transport at room temperature is acceptable. 


Transport Container
Plastic screw-cap vial

Transport Temperature
Refrigerated (cold packs)


Specimen Stability

Room temperature: 4 days

Refrigerated: 7 days

Frozen: 30 days



Methodology
Immunofluorescence Assay (IFA)

Reference Range
ANA Screen Negative
ANA Titer  
<1:40 Negative
1:40-1:80 Low antibody level
>1:80 Elevated antibody level


Clinical Significance
Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE.

Performed By
Quest Diagnostics Nichols Institute



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.