Protime

Test Code
PT


Alias/See Also
INR
Prothrombin time
PT


Preferred Specimen
 One full unopened 3.2% sodium citrate (light blue-top) tube.


Minimum Volume
 Ratio of blood to citrate is critical.  • 0.5 mL frozen plasma


Other Acceptable Specimens
 1 mL frozen plasma: for heparinized patients or if testing will be delayed longer than 24 hours, centrifuge specimen within 1 hour of collection at 2500-3500 rpm. Transfer plasma to a plastic screw-cap vial and freeze at -20° C immediately. Ship on dry ice.


Instructions
 Correct ratio of blood to citrate is critical. Do not overfill or underfill collection tube. Mix by inversion 4 times. For non-heparinized patients, maintain specimen at room temperature. Do not uncap. Stable 24 hours at room temperature.


Transport Container
 3.2% sodium citrate (light blue-top) tube


Transport Temperature
 Room temperature


Specimen Stability
 Unopened light blue-top tube
Room temperature: 24 hours
Refrigerated: Unacceptable
Frozen: Unacceptable

Separated platelet poor plasma
Room temperature: 2 hours
Refrigerated: 4 hours
Frozen -20° C: 14 days
Frozen -70° C: 6 months


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
 Gross hemolysis; Gross lipemia; Improper blood to citrate ratio; Clotted; High hematocrits


Setup Schedule
24/7


Report Available
Same day




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.