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Culture, Blood
MessageFollow proper site cleansing procedure.
Test Code
BLC,BLC2
Includes
Aerobic culture, anaerobic culture. If culture is positive, identification will be performed at an additional charge. Antibiotic susceptibilities are only performed when appropriate.
Preferred Specimen
Adults: 5-10 mL of blood in a BD BACTEC® Plus Aerobic/F (silver label/gray cap) and 4-7 mL in an Anaerobic/F bottle (yellow label and cap)
Neonates and Children 1 to 10 years: 1-3 mL of blood in a BACTEC Peds Plus®/F bottle (pink cap and label)
Neonates and Children 1 to 10 years: 1-3 mL of blood in a BACTEC Peds Plus®/F bottle (pink cap and label)
Minimum Volume
BACTEC® Plus Aerobic/F and Anaerobic/F bottles: 3 mL blood/bottle. BACTEC® Peds Plus® /F bottles: 0.5 mL blood/bottle is acceptable, but 1 mL is preferred.
Other Acceptable Specimens
None
Instructions
Do not submit specimens in SPS, heparin, EDTA, and sodium citrate tubes. Do not refrigerate.
Transport Container
BacT/ALERT FA PLUS (Pale green) and BacT/ALERT FN PLUS (Orange) bottles, BacT/ALERT PF PLUS (Yellow) bottle
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Blood specimens collected in EDTA, sodium citrate or anticoagulants such as SPS or heparin
Methodology
Culture using automated continuous monitoring system
Setup Schedule
24/7
Report Available
3 days
Clinical Significance
Bacterial sepsis constitutes one of the most serious infectious diseases. The detection of microorganisms in a patient's blood has importance in the diagnosis and prognosis of endocarditis, septicemia, or chronic bacteremia.
