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Bilirubin, Direct
Test CodeDBIL
Preferred Specimen
1 mL serum - protected from light
Minimum Volume
0.5 mL
Other Acceptable Specimens
Heparinized plasma
Instructions
Collect neonatal specimens is amber tube.
Transport Container
Spun serum separator tube (SST) - protected from light
Transport Temperature
Refrigerated (cool packs)
Specimen Stability
Refrigerated: 24 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Anticoagulants other than heparin
Methodology
Spectrophotometry (SP)
Setup Schedule
24/7
Report Available
Same day
Reference Range
0.0-0.3 mg/dL
Clinical Significance
Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder obstruction. The assessment of direct bilirubin is helpful in the differentiation of hepatic disorders. The increase in total bilirubin associated with obstructive jaundice is primarily due to the direct (conjugated) fraction. Both direct and indirect bilirubin are increased in the serum with hepatitis.