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Alkali Denaturation Test (APT and Downey)
Test CodeAPT
Alias/See Also
APT Downey
Preferred Specimen
2 mL of a random, bloody stool submitted in a plastic leak-proof container
Minimum Volume
1 mL
Other Acceptable Specimens
2 mL bloody vomitus or bloody mucus, submitted in a plastic, leak-proof container
Instructions
Note: The specimen requirement for this test is a grossly bloody (red) nontarry stool (or bloody vomitus or bloody mucus). If the specimen submitted was not bloody and we were unable to elute any red color from it, the test cannot be performed and will be cancelled
Transport Container
Plastic leak-proof container
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 1 hour
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen
Methodology
Colorimetric (C)
Setup Schedule
24/7
Report Available
Same day
Reference Range
Negative for fetal blood
Clinical Significance
The Alkali Denaturation Test (APT) is useful in distinguishing between newborn swallowing mother's blood and fetal gastrointestinal bleeding (Melena neonatorum).
The APT test is used to determine whether blood in the diaper, vomitus, or feces of a neonate contains predominantly the baby's or the mother's blood. It is a crude, qualitative method based on resistance of hemoglobin F to alkali denaturation.
The APT test is used to determine whether blood in the diaper, vomitus, or feces of a neonate contains predominantly the baby's or the mother's blood. It is a crude, qualitative method based on resistance of hemoglobin F to alkali denaturation.
