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Kleihauer,Fetal Hgb/Interp
Test CodeKLEIW
Alias/See Also
Fetal Stain
CPT Codes
85460
Preferred Specimen
WHOLE BLOOD, EDTA
Minimum Volume
1 ml
Instructions
Maternal blood should be collected as soon after delivery as possible.
Transport Container
LAV
Transport Temperature
Room Temp, or 2-8 degrees C
Specimen Stability
48 hours at 4-8 degrees C; 24 hours at Room Temp
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Cord Blood or any sample >24 hours old. clotted samples or fibrin strands are also rejected
Methodology
Manual with Acid Elution technique
Setup Schedule
Daily on Receipt
Report Available
Upon Completion of Analysis
Limitations
lipemia, icterus, cold agglutinin
Reference Range
0.0%
Clinical Significance
Hemoglobin F is a normal hemoglobin in infants. HgbF may also appear elevated in obstetric patients in which a large feto-maternal bleed is suspected. In such cases, a dose of Rhogam may be indicated, in appropriate patients, to suppress the formation of antibodies by the mother. Intra-uterine transfusion is another occasion when Kleihauer stain may be requested. Pre and post samples are collected to look for a drop in results indicating the transfusion was successful.