Hexagonal Phospholipid Neut.

Test Code

HPN

CPT Codes
85598

Preferred Specimen

1 blue

Minimum Volume

Blue tops must be filled to Minimum Fill Indicator Line on tube.

Other Acceptable Specimens

none

Instructions

This is a reflex test in the Lupus Anticoagulant panel. Outreach clients should double-spin the blue top plasma, remove the plasma from the RBC button, and freeze in a polypropylene tube.

Transport Container

Blue, polypropylene tube

Transport Temperature

Blue - RT Polypropylene tube - Frozen

Specimen Stability

Whole blood - 4 hours at RT Separated plasma - 8 hours at RT or 1 month frozen

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Sample must be free of clots and hemolysis.

Methodology
Clotting Time Measurement

Setup Schedule

Monday & Thursday 0600-1300

Report Available

Upon Completion of Analysis

Reference Range

the reference time is the delta T cut-off time in seconds. the delta T set for this laboratory is 9 seconds.

< 9 seconds = Negative

> or = 9 seconds = Positive

Qualitative Result Only Reported

Clinical Significance

Lupus anticoagulants (LA) are immunoglobulins that generally interfere with phospholipid-dependent in vitro coagulation tests, the most frequently used being the activated partial thromboplastin time (APTT) assay. The common association of LA with recurrent spontaneous abortions and idiopathic thromboembolic diseases underlies the importance of diagnostic tests capable of distinguishing these LA antibodies from anti-factor antibodies, factor deficiencies and heparin, since all four conditions may cause a prolongation of the APTT test. The diagnosis of LA is often difficult because of variable reagent sensitivity and the intrinsic heterogeneity of LA. In practice, factor deficient plasmas are easily identified, since the addition of normal plasma restores normal in vitro clotting time. However, the APTT test alone cannot provide clear-cut differentiation between LA antibody-containing plasmas and those that contain anti-factor antibodies and/or heparin.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.