| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Alpha-Fetoprotein (AFP), Single Marker Screen, Maternal, Serum
MessageTest Code
AFPSM
Alias/See Also
Epic: LAB692
Mayo: MAFP1
AFP Maternal Screening
AFP Neural Tube Defects
Maternal Screening, AFP Single Marker
Mayo: MAFP1
AFP Maternal Screening
AFP Neural Tube Defects
Maternal Screening, AFP Single Marker
CPT Codes
82105
Preferred Specimen
Specimen Type: Serum
Collection Container: Serum gel
Specimen Volume: 1 mL
Minimum Volume
0.5 mL
Other Acceptable Specimens
Collection Container: Red top
Instructions
- Do not collect specimen after amniocentesis as this could affect results.
- Centrifuge and aliquot serum into plastic vial within 2 hours of collection.
- Collect blood between 15 weeks, 0 days and 22 weeks, 6 days.
- Initial or repeat testing is determined in the laboratory at the time of report and will be reported accordingly. To be considered a repeat test for the patient, the testing must be within the same pregnancy and trimester, with interpretable results for the same test, and both tests are performed at Mayo Clinic.
Transport Container
Plastic vial
Specimen Stability
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 90 days | ||
| Ambient | 7 days |
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Methodology
Two-Site Immunoenzymatic (Sandwich) Assay
Setup Schedule
Monday through Friday
Report Available
4 to 6 days
Limitations
CAUTIONS
Race, weight, smoking, multiple fetus pregnancy, and insulin-dependent diabetes (IDD) may affect marker concentrations. Black mothers tend to have higher alpha-fetoprotein (AFP) levels but lower risk of neural tube defects and are assigned to a separate AFP median set. Multiple of the medians (MoM) are adjusted for maternal weight (to account for dilution effects in heavier mothers). The AFP is adjusted upward in IDD to account for lower values in diabetic pregnancies. Smoking results in higher second trimester maternal serum AFP. MoM are adjusted accordingly to account for serum AFP differences in smokers.
The screen results are dependent on accurate information for gestation, race, IDD, and weight. Inaccurate information can lead to significant alterations in the estimated risk. In particular, erroneous assessment of gestational age can result in false-positive or false-negative screen results. Because of its increased accuracy, the determination of gestational age by ultrasound is recommended, when possible, rather than by last menstrual period.
A screen-negative result does not guarantee the absence of fetal defects. A screen-positive result does not provide a diagnosis but indicates that further diagnostic testing should be considered (an unaffected fetus may have screen-positive result for unknown reasons).
Valid measurements of AFP in maternal serum cannot be made after amniocentesis.
Triplet and higher multiple pregnancies cannot be interpreted.
Each center offering maternal serum screening to patients should establish a standard screening protocol, which provides pre- and post-screening education and appropriate follow-up for screen-positive results.
In rare cases, some individuals can develop antibodies to mouse or other animal antibodies (often referred to as human anti-mouse antibodies [HAMA] or heterophile antibodies), which may cause interference in some immunoassays. Caution should be used in interpretation of results and the laboratory should be alerted if the result does not correlate with the clinical presentation.
Race, weight, smoking, multiple fetus pregnancy, and insulin-dependent diabetes (IDD) may affect marker concentrations. Black mothers tend to have higher alpha-fetoprotein (AFP) levels but lower risk of neural tube defects and are assigned to a separate AFP median set. Multiple of the medians (MoM) are adjusted for maternal weight (to account for dilution effects in heavier mothers). The AFP is adjusted upward in IDD to account for lower values in diabetic pregnancies. Smoking results in higher second trimester maternal serum AFP. MoM are adjusted accordingly to account for serum AFP differences in smokers.
The screen results are dependent on accurate information for gestation, race, IDD, and weight. Inaccurate information can lead to significant alterations in the estimated risk. In particular, erroneous assessment of gestational age can result in false-positive or false-negative screen results. Because of its increased accuracy, the determination of gestational age by ultrasound is recommended, when possible, rather than by last menstrual period.
A screen-negative result does not guarantee the absence of fetal defects. A screen-positive result does not provide a diagnosis but indicates that further diagnostic testing should be considered (an unaffected fetus may have screen-positive result for unknown reasons).
Valid measurements of AFP in maternal serum cannot be made after amniocentesis.
Triplet and higher multiple pregnancies cannot be interpreted.
Each center offering maternal serum screening to patients should establish a standard screening protocol, which provides pre- and post-screening education and appropriate follow-up for screen-positive results.
In rare cases, some individuals can develop antibodies to mouse or other animal antibodies (often referred to as human anti-mouse antibodies [HAMA] or heterophile antibodies), which may cause interference in some immunoassays. Caution should be used in interpretation of results and the laboratory should be alerted if the result does not correlate with the clinical presentation.
Reference Range
REFERENCE VALUES
NEURAL TUBE DEFECTS:
An alpha-fetoprotein (AFP) multiple of the median (MoM) <2.5 is reported as screen negative.
AFP MoM > or =2.5 (singleton and twin pregnancies) are reported as screen positive.
An interpretive report will be provided.
INTERPRETATION
Neural tube defects:
A screen-negative result indicates that the calculated alpha-fetoprotein (AFP) multiple of the median (MoM) falls below the established cutoff of 2.50 MoM. A negative screen does not guarantee the absence of neural tube defects (NTD).
A screen-positive result indicates that the calculated AFP MoM is 2.50 or greater and may indicate an increased risk for open NTD. The actual risk depends on the level of AFP and the individual's pretest risk of having a child with NTD based on family history, geographical location, maternal conditions such as diabetes and epilepsy, and use of folate prior to conception. A screen-positive result does not infer a definitive diagnosis of a NTD but indicates that further evaluation should be considered. Approximately 80% of pregnancies affected with an open NTD have elevated AFP MoM values greater than 2.50.
Follow up:
Upon receiving maternal serum screening results, all information used in the risk calculation should be reviewed for accuracy (ie, weight, diabetic status, gestational dating). If any information is incorrect the laboratory should be contacted for a recalculation of the estimated risks.
Screen-negative results typically do not warrant further evaluation.
Ultrasound is recommended to confirm dates for NTD screen-positive results. If ultrasound yields new dates that differ by at least 7 days, a recalculation should be considered. If dates are confirmed, high-resolution ultrasound and amniocentesis (including amniotic fluid AFP and acetylcholinesterase measurements for NTD) are typically offered.
NEURAL TUBE DEFECTS:
An alpha-fetoprotein (AFP) multiple of the median (MoM) <2.5 is reported as screen negative.
AFP MoM > or =2.5 (singleton and twin pregnancies) are reported as screen positive.
An interpretive report will be provided.
INTERPRETATION
Neural tube defects:
A screen-negative result indicates that the calculated alpha-fetoprotein (AFP) multiple of the median (MoM) falls below the established cutoff of 2.50 MoM. A negative screen does not guarantee the absence of neural tube defects (NTD).
A screen-positive result indicates that the calculated AFP MoM is 2.50 or greater and may indicate an increased risk for open NTD. The actual risk depends on the level of AFP and the individual's pretest risk of having a child with NTD based on family history, geographical location, maternal conditions such as diabetes and epilepsy, and use of folate prior to conception. A screen-positive result does not infer a definitive diagnosis of a NTD but indicates that further evaluation should be considered. Approximately 80% of pregnancies affected with an open NTD have elevated AFP MoM values greater than 2.50.
Follow up:
Upon receiving maternal serum screening results, all information used in the risk calculation should be reviewed for accuracy (ie, weight, diabetic status, gestational dating). If any information is incorrect the laboratory should be contacted for a recalculation of the estimated risks.
Screen-negative results typically do not warrant further evaluation.
Ultrasound is recommended to confirm dates for NTD screen-positive results. If ultrasound yields new dates that differ by at least 7 days, a recalculation should be considered. If dates are confirmed, high-resolution ultrasound and amniocentesis (including amniotic fluid AFP and acetylcholinesterase measurements for NTD) are typically offered.
Clinical Significance
USEFUL FOR
Prenatal screening for open neural tube defect
CLINICAL INFORMATION
Alpha-fetoprotein (AFP) is a fetal protein that is initially produced in the fetal yolk sac and liver. A small amount is produced by the gastrointestinal tract. By the end of the first trimester, nearly all AFP is produced by the fetal liver. The concentration of AFP peaks in fetal serum between 10 to 13 weeks. Fetal AFP diffuses across the placental barrier into the maternal circulation. A small amount also is transported from the amniotic cavity.
The AFP concentration in maternal serum rises throughout pregnancy, from the nonpregnancy level of 0.2 ng/mL to about 250 ng/mL at 32 weeks gestation. If the fetus has an open neural tube defect (NTD), AFP is thought to leak directly into the amniotic fluid causing unexpectedly high concentrations of AFP. Subsequently, the AFP reaches the maternal circulation, producing elevated serum levels. Other fetal abnormalities such as omphalocele, gastroschisis, congenital kidney disease, esophageal atresia, and other fetal distress situations (eg, threatened abortion and fetal demise) also may result in maternal serum AFP elevations. Increased maternal serum AFP concentrations also may be seen in multiple pregnancies and in unaffected singleton pregnancies in which the gestational age has been underestimated.
Lower maternal serum AFP concentrations have been associated with an increased risk for genetic conditions such as trisomy 21 (Down syndrome) and trisomy 18 (Edwards syndrome). Risks for these syndrome disorders are only provided with the use of multiple marker screening (QUAD1 / Quad Screen [Second Trimester] Maternal, Serum).
Measurement of maternal serum AFP values is a standard tool used in obstetrical care to identify pregnancies that may have an increased risk for NTD. The screen is performed by measuring AFP in maternal serum and comparing this value to the median AFP value in an unaffected population to obtain a multiple of the median (MoM). The laboratory has established a MoM cutoff of 2.5, which classifies each screen as either screen-positive or screen-negative. A screen-positive result indicates that the value obtained exceeds the established cutoff. A positive screen does not provide a diagnosis but indicates that further evaluation should be considered.
Performing Laboratory
Mayo Clinic Laboratories - Rochester
3050 Superior Drive NW
Rochester, MN 55901
Additional Information
Alpha-Fetoprotein (AFP), Single Marker Screen, Maternal, Serum
Last Updated: August 17, 2023
Last Review: N. Wolford, August 4, 2023