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Ascorbic Acid (Vitamin C), Plasma
MessageShip specimen in amber vial to protect from light.
Test Code
ASCAD
Alias/See Also
Epic: LAB8285
Mayo: VITC
Mayo: VITC
CPT Codes
82180
Preferred Specimen
Specimen Type: Plasma Heparin
Collection Container: Green top (sodium or lithium heparin) or plasma gel separator (PST)
Specimen Volume: 1 mL
Patient Preparation
Fasting overnight (12-14 hours) (infants-collect prior to next feeding). Water can be taken as needed.
Minimum Volume
0.5 mL
Instructions
- Immediately place specimen on wet ice and process within 4 hours of collection.
- Centrifuge at 4 degrees C, aliquot plasma into amber vial to protect from light, and freeze immediately.
Transport Container
Plastic vial
Specimen Stability
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Heparin | Frozen | 14 days | LIGHT PROTECTED |
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Methodology
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Portions of this test are covered by patents held by Quest Diagnostics
Setup Schedule
Monday through Friday
Report Available
3 to 5 days
Limitations
CAUTIONS
Testing of nonfasting specimens or the use of vitamin supplementation can result in elevated plasma vitamin concentrations. Reference values were established in patients who were fasting.
After consuming vitamin C, plasma values rapidly rise within 1 to 2 hours and reach peak concentration within 3 to 6 hours after ingestion.
Testing of nonfasting specimens or the use of vitamin supplementation can result in elevated plasma vitamin concentrations. Reference values were established in patients who were fasting.
After consuming vitamin C, plasma values rapidly rise within 1 to 2 hours and reach peak concentration within 3 to 6 hours after ingestion.
Reference Range
REFERENCE VALUES
0.4-2.0 mg/dL
INTERPRETATION
Values below 0.2 mg/dL indicate significant deficiency.
Values greater than or equal to 0.2 mg/dL and less than 0.4 mg/dL are consistent with a moderate risk of deficiency due to inadequate tissue stores.
Values of 0.4 to 2.0 mg/dL indicate adequate supply.
The actual level at which vitamin C is excessive has not been defined. Values above 3.0 mg/dL are suggestive of excess intake. Whether vitamin C in excess is indeed toxic continues to be uncertain. However, limited observations suggest that this condition may induce uricosuria and, in individuals with glucose-6-phosphate dehydrogenase deficiency, may induce increased red blood cell fragility.
0.4-2.0 mg/dL
INTERPRETATION
Values below 0.2 mg/dL indicate significant deficiency.
Values greater than or equal to 0.2 mg/dL and less than 0.4 mg/dL are consistent with a moderate risk of deficiency due to inadequate tissue stores.
Values of 0.4 to 2.0 mg/dL indicate adequate supply.
The actual level at which vitamin C is excessive has not been defined. Values above 3.0 mg/dL are suggestive of excess intake. Whether vitamin C in excess is indeed toxic continues to be uncertain. However, limited observations suggest that this condition may induce uricosuria and, in individuals with glucose-6-phosphate dehydrogenase deficiency, may induce increased red blood cell fragility.
Clinical Significance
USEFUL FOR
Identifying vitamin C deficiency
CLINICAL INFORMATION
Vitamin C, also known as L-ascorbic acid or simply ascorbic acid, is a water-soluble vitamin that is naturally present in some foods, added to others, and available as a dietary supplement. Humans, unlike most animals, are unable to synthesize vitamin C endogenously, so it is an essential dietary component. Vitamin C is required for the enzymatic amidation of neuropeptides, production of adrenal cortical steroid hormones, promotion of the conversion of tropocollagen to collagen, and metabolism of tyrosine and folate. It also plays a role in lipid and vitamin metabolism and is a powerful reducing agent or antioxidant. Specific actions include activation of detoxifying enzymes in the liver; antioxidation, interception and destruction of free radicals; preservation and restoration of the antioxidant potential of vitamin E; and blockage of the formation of carcinogenic nitrosamines. In addition, vitamin C appears to function in a variety of other metabolic processes in which its role has not been well characterized.
Prolonged deficiency of vitamin C leads to the development of scurvy, a disease characterized by an inability to form adequate intercellular substance in connective tissues. This results in the formation of swollen, ulcerative lesions in the gums, mouth, and other tissues that are structurally weakened. Early symptoms may include weakness, easy fatigue and listlessness, as well as shortness of breath, and aching joints, bones, and muscles.
The need for vitamin C can be increased by the use of aspirin, oral contraceptives, tetracycline, and a variety of other medications. Psychological stress and advancing age also tend to increase the need for vitamin C. Among older adults, lack of fresh fruit and vegetables often adds vitamin C depletion to the inherently increased need, with development of near-scurvy status
Identifying vitamin C deficiency
CLINICAL INFORMATION
Vitamin C, also known as L-ascorbic acid or simply ascorbic acid, is a water-soluble vitamin that is naturally present in some foods, added to others, and available as a dietary supplement. Humans, unlike most animals, are unable to synthesize vitamin C endogenously, so it is an essential dietary component. Vitamin C is required for the enzymatic amidation of neuropeptides, production of adrenal cortical steroid hormones, promotion of the conversion of tropocollagen to collagen, and metabolism of tyrosine and folate. It also plays a role in lipid and vitamin metabolism and is a powerful reducing agent or antioxidant. Specific actions include activation of detoxifying enzymes in the liver; antioxidation, interception and destruction of free radicals; preservation and restoration of the antioxidant potential of vitamin E; and blockage of the formation of carcinogenic nitrosamines. In addition, vitamin C appears to function in a variety of other metabolic processes in which its role has not been well characterized.
Prolonged deficiency of vitamin C leads to the development of scurvy, a disease characterized by an inability to form adequate intercellular substance in connective tissues. This results in the formation of swollen, ulcerative lesions in the gums, mouth, and other tissues that are structurally weakened. Early symptoms may include weakness, easy fatigue and listlessness, as well as shortness of breath, and aching joints, bones, and muscles.
The need for vitamin C can be increased by the use of aspirin, oral contraceptives, tetracycline, and a variety of other medications. Psychological stress and advancing age also tend to increase the need for vitamin C. Among older adults, lack of fresh fruit and vegetables often adds vitamin C depletion to the inherently increased need, with development of near-scurvy status
Performing Laboratory
Mayo Clinic Laboratories - Rochester
3050 Superior Drive NW
Rochester, MN 55901
Additional Information
Ascorbic Acid (Vitamin C), Plasma
Last Updated: July 3, 2023
Last Review: N. Wolford, July 3, 2023