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Ova and Parasites, Concentrate and Permanent Smear [681X]
Test CodeAlias/See Also
LAB6872
CPT Codes
87177, 87209
Includes
Preferred Specimen
Patient Preparation: Interfering substances-bismuth, barium (wait 7-10 days), antimicrobial agents (wait 2 weeks), gall bladder dye (wait 3 weeks after procedure).
Specimen Type: Preserved stool
Collection Container/Type
Preferred: Total-Fix transport vial
Acceptable: 10% formalin AND Polyvinyl Alcohol Transport (PVA) vials, Sodium Para-Pak SVT transport vial
Specimen Volume: 10 grams or 10 mL
Collection Instructions:
- Place fresh stool in preservative vials within 30 minutes of collection.
- Add stool to bring the liquid level to the ""fill to here"" line on the vial.
- Mix well.
- Send specimens at room temperature in the same shipping container.
Additional Information:
- If parasite infestation is strongly suspected, collet at least 3 stool specimens every other day, since a single specimen can be negative. If Giardia is strongly suspected, see CRYGI, Cryptosporidium/Giardia Antigen
Specimen Type: BAL
Collection Container/Type
Preferred: Sterile container
Acceptable: 10% formalin
Specimen Volume: 10 mL
Collection Instructions:
- The specimen should be a deep expectorated sputum, preferably collected in the early morning.
- A 24-hour sputum collection is also accpetable.
- Submit in a sterile screw-capped container, unpreserved or in 10% formalin to increase stability.
Specimen Type: Urine
Collection Container/Type: Sterile container
Specimen Volume: 25 mL
Collection Instructions:
- Urine may be submitted unpreserved for exam for Schistosoma.
- Collect at mid-day.
- Peak egg secretion occurs between noon and 3 p.m.
- Do not submit first morning specimen.
- In patient with hematuria, eggs may be found trapped in blood and mucous in the terminal portion (last-voided portion) of the urine.
Patient Preparation
Interfering substances-bismuth, barium (wait 7-10 days), antimicrobial agents (wait 2 weeks), gall bladder dye (wait 3 weeks after procedure).
Minimum Volume
5 grams or 5 mL of stool
10 mL urine (unpreserved)
2 mL sputum or BAL
Other Acceptable Specimens
Instructions
Place fresh stool in 10% formalin transport vial, as well as PVA transport medium or single Total-Fix® vial, within 30 minutes of collection. Add stool to bring the liquid level to the "fill to here" line on the vial. Mix well. Send specimen(s) at room temperature in the same shipping container. If parasite infestation is strongly suspected, collect at least 3 stool specimens every other day, since a single specimen can be negative. If Giardia is strongly suspected, please see Giardia Antigen, EIA, Stool, Test Code 8625.
Urine may be submitted unpreserved for exam for Schistosoma. Collect at mid-day. Peak egg secretion occurs between noon and 3 p.m. Do not submit first morning specimen. In patients with hematuria, eggs may be found trapped in blood and mucous in the terminal portion (last-voided portion) of the urine specimen.
Sputum: The specimen should be a deep expectorated sputum, preferably collected in the early morning. A 24-hour sputum collection is also acceptable. Submit in a sterile screw-capped container, unpreserved or in 10% formalin to increase stability.
Transport Temperature
Unpreserved specimens: Refrigerated (cold packs)
Specimen Stability
Stool (preserved)
Room temperature: 6 months
Refrigerated: 6 months
Frozen: Unacceptable
Urine, Sputum, BAL (unpreserved)
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen: Unacceptable
Sputum, BAL (preserved)
Room temperature: 6 months
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unpreserved stool • Specimens containing barium •
Stool preserved in medium other than parasitology fixative •
Received frozen • Stool submitted in expired transport vial
• Preserved urine • Unpreserved sputum and urine
received room temperature or frozen • Liver abscess or
aspirate • Stool submitted in EcoFix® transport vial
Methodology
Microscopic Examination of Concentrate • Permanent Stained
Smear
Setup Schedule
Daily
Report Available
5-7 days
Reference Range
Clinical Significance
Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153
Last Updated: February 20, 2023