Nonesterified Fatty Acids (Free Fatty Acids)

Test Code
NFA


Alias/See Also

LAB986



CPT Codes
<p>82725</p>

Preferred Specimen

Patient Preparation: Overnight fasting is preferred


 


Submit only 1 of the following specimens:


 


Specimen Type: Serum


Collection Container/Type


Preferred: Serum gel


Acceptable: Red topPlain, royal blue-top Vacutainer plastic trace element blood collection tube


Submission Container/Tube: Plastic vial


Specimen Volume: 1 mL


Collection Instructions: Serum or plasma should be removed from cells immediately after collection.


 


Specimen Type: Plasma


Collection Container/Type


Preferred: Lavender top (EDTA)


Acceptable: Plain, royal blue-top Vacutainer plastic trace element blood collection tubeLight blue top (citrate)Gray top (Sodium fluoride)White top (PPT potassium EDTA)


Submission Container/Tube: Plastic vial


Specimen Volume: 1 mL


Collection Instructions: Serum or plasma should be removed from cells immediately after collection.



Patient Preparation
Overnight fasting is preferred

Minimum Volume

0.3 mL



Other Acceptable Specimens
Serum collected in: no additive (royal blue-top) tube • Plasma collected in: an EDTA (lavender or royal blue-top), 3.2% sodium citrate (light blue-top), sodium fluoride or potassium oxalate (gray-top), or PTH-related protein (special collection tube), ACTH (lavender-top), or PPT potassium EDTA (white-top) tube


Instructions
Serum or plasma should be removed from cells immediately after collection.


Transport Temperature
Frozen


Specimen Stability

Room temperature: Unacceptable


Refrigerated: 2 hours


Frozen: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received room temperature • Received refrigerated • Heparin Plasma and patients on heparin therapy



Methodology

Spectrophotometry (SP) • Enzymatic



Setup Schedule
Set up: Tues, Thurs; Report available: 4-9 days


Report Available

4-9 days



Reference Range
Newborns <2.3 mmol/L
1-12 Months 0.50-1.60 mmol/L
1-7 Years 0.60-1.50 mmol/L
8-17 Years 0.20-1.10 mmol/L
Adults 0.07-0.88 mmol/L


Clinical Significance
The Nonesterified Fatty Acids (Free Fatty Acids) assay is useful in evaluating lipolysis and fatty acid oxidation. Nonesterified Fatty Acids may be useful in evaluating patients with hypoglycemia and for nutritional assessment.


Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042




Last Updated: February 17, 2023


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.