HIV-2 DNA/RNA, Qualitative Real-Time PCR

Test Code
HIV2P


CPT Codes
<p>87538</p>

Preferred Specimen

Specimen Type: EDTA Whole Blood


Collection Container/Type: Lavender top (EDTA)


Specimen Volume: 1 mL



Minimum Volume

0.4 mL



Transport Temperature
Room temperature


Specimen Stability

Room temperature: 7 days

Refrigerated: 14 days

Frozen: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis • Heparinized whole blood



Methodology

Real-Time Polymerase Chain Reaction

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.



FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 2-3 days


Report Available

2 - 3 days



Reference Range
Not detected


Clinical Significance
Infection with Human Immunodeficiency Virus type 2 (HIV-2) is currently diagnosed by the presence of antibodies to HIV-2, the detection of specific HIV-2 antigens or the ability to culture HIV-2 from blood, fluid or tissue of infected persons. The Polymerase Chain Reaction (PCR) technique, on the other hand, allows for the exponential enzymatic amplification of selected HIV-2 DNA sequences present in clinical specimens. The amplified fragments are then detected by hybridization to an HIV-2 specific labeled probe. As a result, the HIV-2 DNA PCR is a highly sensitive and specific method to detect the presence of HIV-2 proviral DNA in clinical specimens.
The diagnosis of infection should not rely solely upon the result of a PCR assay. A positive result should be considered in conjunction with clinical presentation and additional clinical tests. A negative PCR result indicates the absence of HIV-2 proviral DNA at detectable levels in the sample tests and does not exclude diagnosis of disease.


Performing Laboratory

Quest Diagnostics Infectious Disease, Inc.
33608 Ortega Highway, Bldg B-West Wing
San Juan Capistrano, 92675-2042




Last Updated: February 17, 2023


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.