A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Parvovirus B19 DNA, Quantitative Real-Time PCR

Test Code
PB19Q


Alias/See Also

LAB3302



CPT Codes
<p>87799</p>

Preferred Specimen

Submit only 1 of the following specimens:


Specimen Type: Plasma

Collection Container/Type

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD), White top (PPT potassium EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

 

Specimen Type: Whole blood

Collection Container/Type

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 1 mL

 

Specimen Type: Amniotic fluid

Collection Container/Type: Sterile container

Submission Container/Tube: Sterile container

Specimen Volume: 1 mL

Collection Instructions: 

  1. Do not centrifuge.
  2. Label specimen as amniotic fluid.

 

Specimen Type: Serum

Collection Container/Type

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL



Minimum Volume

0.3 mL



Transport Temperature
Whole blood: Refrigerated (cold packs)
All other samples: Frozen


Specimen Stability

Whole blood


Room temperature: 48 hours


Refrigerated: 7 days


Frozen: Unacceptable


 


All other samples


Room temperature: 48 hours


Refrigerated: 7 days


Frozen: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolyzed whole blood • Heparin plasma or whole blood



Methodology

Real-Time Polymerase Chain Reaction

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.



FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Monday-Sunday Morning
Report available: Next Day


Report Available

1-2 days



Reference Range
Parvovirus B19 DNA, QN PCRNot Detected (copies/mL)
Parvovirus B19 DNA, QN PCRNot Detected (Log copies/mL)


Clinical Significance
Parvovirus B19 is the cause of an infection known as "Fifth Disease". This infection affects children primarily and causes a rash on the face, trunk, and limbs. Joint pain and swelling is more common in adults. Although a portion of those affected have only mild disease, patients with sickle cell anemia, or similar types of chronic anemia, can suffer from severe acute anemia. Infection during pregnancy can lead to severe complications for the fetus. Infections in immunocompromised patients may lead to serious complications including nephropathy, myocarditis, and persistent anemia. Although serologic detection of circulating antibodies (Parvovirus B-19 IgM & IgG) is useful to determine recent exposure to the virus, DNA testing provides the most reliable evidence of a persistent infection. Quantitative results of Parvovirus B19 viral load may be useful in determining the response to therapy (usually IVIG) and may be beneficial in assessing the risk for nosocomial transmission.


Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153


Last Updated: February 17, 2023


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.