A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Herpes Simplex Virus, Type 1 and 2 DNA, Qualitative Real Time PCR

Test Code
HS12P


Alias/See Also
Epic: LAB917
Quest: 34257


CPT Codes
<p>87529 (x2)</p>

Preferred Specimen

Specimen
Type: (Fluid) CSF, pleural fluid, amniotic fluid,
pericardial fluid


Collection
Container/Type: Sterile container


Submission
Container/Tube: Sterile container


Specimen
Volume: 1 mL


Collection
Instructions: 



  1. Collect in a sterile
    leak proof-container and store refrigerated or frozen.


 


Specimen
Type: Serum


Collection
Container/Type: Serum gel


Submission
Container/Tube: Plastic vial


Specimen
Volume: 1 mL


Collection
Instructions: 



  1. Collect blood in
    sterile tubes with no anticoagulants; serum separator tubes
    (SST®) are recommended. Transfer serum into sterile leak-proof
    container and store refrigerated or frozen.


 


Specimen
Type: Whole Blood


Collection
Container/Type



Preferred: Lavender top (EDTA)



Acceptable: Yellow top (ACD)


Specimen
Volume: 1 mL


 


Specimen
Type: Plasma


Collection
Container/Type



Preferred: Lavender top (EDTA)



Acceptable: Yellow top (ACD)


Submission
Container/Tube: Plastic vial


Specimen
Volume: 1 mL


 


Specimen
Type: Swab


Collection
Container/Type



Preferred: Viral transport media, VCM, M4



Acceptable: Aptima Unisex Collection device
(white label)


Specimen
Volume: Swab


Collection
Instructions: 



  1. Swab a lesion and
    place swab in a viral transport media, VCM, M4 or APTIMA®
    Unisex Collection device (white label).


 


Specimen
Type: Vitreous fluid


Collection
Container/Type: Sterile container


Submission
Container/Tube: Sterile container


Specimen
Volume: 0.5 mL


 


Specimen
Type: BAL, Bronchial Wash


Collection
Container/Type: Sterile container


Submission
Container/Tube: Sterile container


Specimen
Volume: 1 mL



Minimum Volume

0.35 mL



Other Acceptable Specimens
Bronchoalveolar lavage or bronchial wash collected in a sterile leak-proof container


Instructions
CSF: Collect in a sterile leak proof-container and store refrigerated or frozen.

Swab: Swab a lesion and place swab in a viral transport media, VCM, M4 or APTIMA® Unisex Collection device (white label).

Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST®) are recommended. Transfer serum into sterile leak-proof container and store refrigerated or frozen.

Bronchial Wash and BAL: Collect in a sterile leak-proof container.


Transport Temperature
Refrigerated (cold packs)


Specimen Stability

APTIMA® Unisex Collection device (white
label)


Room temperature: 14 days


Refrigerated: 14 days


Frozen: 30 days


 


All other container types


Room temperature: 48 hours


Refrigerated: 7 days


Frozen: 30 days (whole blood unacceptable)



Methodology

Real-Time Polymerase Chain Reaction

 

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.



FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Daily



Report Available

1 day



Reference Range
HSV 1 DNA Not detected
HSV 2 DNA Not detected


Clinical Significance
DNA testing is analytically more sensitive than culture, especially in patients with encephalitis or meningitis. DNA testing may be useful in diagnosis of infection in neonates. Neonates who have been exposed to HSV can develop disseminated infection and encephalitis. Encephalitis is usually due to HSV I whereas meningitis is usually due to HSV II. DNA testing provides reliable means to define the type.


Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153


Last Updated: April 27, 2023


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.