A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Cytomegalovirus DNA, Qualitative Real-Time PCR

Test Code
CMDDQ


Alias/See Also

LAB3039



CPT Codes
<p>87496</p>

Preferred Specimen

Submit only 1 of the following specimens

 

Specimen Type: Whole Blood

Collection Container/Tube: Lavender top (EDTA) or yellow top (ACD solution A or B)

Specimen Volume: 1 mL

Collection Instructions: 

  1. Collect whole blood in sterile tubes containing EDTA or ACD as anticoagulant.
  2. Store refrigerated.
  3. Do not freeze whole blood.

 

Specimen Type: Plasma or Serum

Collection Container/Type

Preferred: Lavender top (EDTA) or white top (EDTA PPT) or yellow top (ACD solution B)

Acceptable: Serum gel 

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: 

  1. Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in Plasma Preparation Tubes (PPTs).
  2. Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection.
  3. Transfer plasma to sterile, plastic, screw-capped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen.
  4. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.

Additional Information:

  • Do not freeze serum gel tubes.

 

Specimen Type: Fluid

Sources: CSF, amniotic fluid supernatant, bronchoalveolar lavage

Collection Container/Type: Sterile container 

Specimen Volume: 1 mL

Collection Instructions: 

  1. Collect in a sterile container.
  2. Store refrigerated or frozen.

 

Specimen Type: Urine

Container/Tube: Sterile container

Specimen Volume: 1 mL

Collection Instructions: Collect a random urine specimen.

 

Specimen Type: Tissue (unfixed)

Collection Container/Type: Sterile container 

Specimen Volume: 3 mm

Collection Instructions: 

  1. Collect in a sterile container
  2. Store frozen.

 

Specimen Type: Eye fluid

Collection Container/Type: Sterile container 

Specimen Volume: 1 mL

Collection Instructions: Call lab

 



Minimum Volume

0.5 mL



Other Acceptable Specimens
Minimum: 3mm3 frozen fresh tissue, submitted in a sterile, plastic, leak-proof container • 1 mL eye fluid submitted in a sterile, leak-proof container (Call Lab)


Instructions
Blood collected in tubes with heparin anticoagulant are not accepted for this test.

Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.

Whole blood: Collect whole blood in sterile tubes containing EDTA or ACD as anticoagulant. Store refrigerated. Do not freeze whole blood.

Serum, CSF, Amniotic Fluid, urine, and bronchoaveolar lavage: Collect in a sterile container and store refrigerated or frozen.

Fresh (unfixed) tissue: Collect in a sterile container and store frozen.

Eye fluid specimens: Call lab


Transport Temperature
Fresh tissue: Frozen
All other sample types: Refrigerated (cold packs)


Specimen Stability

Whole Blood


Room temperature: 48 hours


Refrigerated: 8 days


Frozen: Unacceptable


 


All Other Samples


Room temperature: 48 hours


Refrigerated: 8 days


Frozen: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Heparinized specimens • Unspun PPT tube



Methodology

Real-Time Polymerase Chain Reaction

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.



FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily a.m.; Report available: Next day


Report Available

Next day



Reference Range
Not detected


Clinical Significance
In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid.


Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153


Last Updated: February 17, 2023


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.