A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Activated Protein C-Resistance

Test Code
ACTPR


Alias/See Also

LAB2917



CPT Codes
<p>85307</p>

Preferred Specimen

Specimen Type: Plasma Na Ct


Collection Container/Type: Light-blue top (3.2% sodium citrate)


Submission Container/Tube: Plastic vial


Specimen Volume: 2 mL


Collection Instructions: 



  1. Draw blood into a light blue (sodium citrate) tube.  Be sure to fill tubes completely.

  2. Mix gently 5 to 6 times (do not over mix).

  3. Centrifuge the sample for 10 minutes.

  4. Using a pipette, aliquot plasma into a plastic vial being careful not disturb the buffy coat.

  5. Centrifuge the plasma sample a second time for 10 minutes

  6. Using a pipette, aliquot the top portion of plasma into a plastic vial.  Be careful not to disturb the sediment at the bottom (leave approx. 0.25 mL of plasma at the bottom).

  7. Label each tube as "Plasma."

  8. Immediately freeze samples


For complete instructions, see Platelet Poor Plasma Instructions.


 



Minimum Volume

1 mL



Instructions
Platelet-poor plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (< 10,000/mcL). Freeze immediately and ship on dry ice.


Transport Temperature
Frozen


Specimen Stability

Room temperature: Unacceptable


Refrigerated: Unacceptable


Frozen: 60 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received room temperature • Received refrigerated • Clotted specimen • Serum • Gross hemolysis



Methodology

RVVT-based Assay (Clot-based)



Setup Schedule
Set up: Mon-Sat; Report available: Next day


Report Available

Next day



Limitations

Expected impact by therapeutic levels (potential interference depends upon drug concentration): Warfarin: no effect; Heparin (UFH or LMWH): no effect; Dabigatran or Argatroban (Thrombin Inhibitors): unable to obtain assay endpoint; Rivaroxaban or Apixaban (Factor Xa Inhibitors): no effect.



Reference Range
≥2.1 Ratio


Clinical Significance
Activated protein C (APC) resistance is the most frequent hereditary defect associated with deep vein thrombosis. Over 95% of the APC resistance phenotype is due to the Factor V Leiden mutation. The APC-Resistance assay is a plasma based functional test for the determination of APC resistance caused by the factor V Leiden mutation.


Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153




Last Updated: February 17, 2023


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.