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Antithrombin III Activity
Test CodeATIII
Alias/See Also
Epic: LAB311
CPT Codes
85300
Preferred Specimen
Specimen Type: Platelet-poor plasma
Collection Container: Light blue top (citrate)
Specimen Volume: 1 mL
Minimum Volume
1 mL
Instructions
- Draw blood into a light blue (sodium citrate) tube. Be sure to fill tubes completely.
- Mix gently 5 to 6 times (do not over mix).
- Centrifuge the sample for 10 minutes.
- Using a pipette, aliquot plasma into a plastic vial being careful not disturb the buffy coat.
- Centrifuge the plasma sample a second time for 10 minutes
- Using a pipette, aliquot the top portion of plasma into a plastic vial. Be careful not to disturb the sediment at the bottom (leave approx. 0.25 mL of plasma at the bottom).
- Label each tube as "Plasma."
- Immediately freeze samples
Transport Container
Plastic vial
Specimen Stability
Room temperature: 4 hours
Refrigerated: Unacceptable
Frozen: 1 month
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples that are short draws (QNS), overfilled, clotted, hemolyzed (hemoglobin greater than 7 g/l), or exceed stability requirements, may yield incorrect results. Also specimens that present with a bilirubin greater than 200 mg/l and /or triglycerides greater than 7.6 g/l , ie. grossly icteric or lipemic.
Setup Schedule
Daily
Report Available
1 day
Reference Range
See laboratory report
Performing Laboratory
Inova Laboratories
2832 Juniper Street
Fairfax, VA 22031
Last Updated: March 6, 2023
Last Review: N. Wolford, March 6, 2023