Hepatitis B Core Total Antibodies

Test Code

HBCT

Alias/See Also

Epic: LAB1242

Preferred Specimen

Specimen Type: Serum
Collection Container: Serum gel
Specimen Volume: 3 mL

Minimum Volume

0.25 mL

Other Acceptable Specimens

Collection Container: Red top

Instructions

Centrifuge and separate cells after clot formation and within 4 hours of collection.

Transport Container

Plastic vial

Specimen Stability

Room temperature: 3 day
Refrigerated: 7 days
Frozen: > 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Unlabeled, mislabeled, wrong tube type, QNS; Hemolysis index >500, bilirubin >20 mg/dL, TP >12 g/dL, Triglycerides >3000 mg/dL

Methodology

Chemiluminescent Microparticle Immunoassay (CMIA).

Setup Schedule

Daily

Report Available

Same day

Reference Range

Non-reactive

Clinical Significance

Chemiluminescent microparticle immunoassay for the qualitative detection of IgG and IgM antibodies to hepatitis B core antigen (anti-HBc). Microparticles coated with recombinant hepatitis B virus core antigen and combined with sample and diluent. The anti-HBc antibodies present in the sample bind to the coated microparticles. After washing, Pre-trigger and trigger solutions are added; the resulting chemiluminescent reaction is measured as relative light units (RLUs). The presence or absence of anti-HBc antibodies in the specimen is determined by comparing the chemiluminescent signal in the reaction to the cutoff signal determined from an active calibration.

Performing Laboratory

Inova Laboratories

2832 Juniper Street

Fairfax, VA 22031

Last Updated: April 10, 2023
Last Review: N. Wolford, April 10, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.