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HIT w/Reflex SRA, UFH
MessageBoth plasma and serum are required.
Test Code
HITSR
Alias/See Also
Epic: LAB15748
Heparin-Induced Thrombocytopenia (HIT)
Low molecular weight heparin
PF4-H
LMWH
Heparin-Induced Thrombocytopenia (HIT)
Low molecular weight heparin
PF4-H
LMWH
Includes
If result is positive, testing will reflex to SRA, Serotonin Release Assay (SRA), UFH at an additional charge.
Preferred Specimen
Specimen Type: Platelet-poor plasma
Collection Container: Light blue top (citrate)
Specimen Volume: 1 mL
Minimum Volume
0.5 mL
Instructions
- Draw blood into a light blue (sodium citrate) tube. Be sure to fill tubes completely.
- Mix gently 5 to 6 times (do not over mix).
- Centrifuge the sample for 10 minutes.
- Using a pipette, aliquot plasma into a plastic vial being careful not disturb the buffy coat.
- Centrifuge the plasma sample a second time for 10 minutes
- Using a pipette, aliquot the top portion of plasma into a plastic vial. Be careful not to disturb the sediment at the bottom (leave approx. 0.25 mL of plasma at the bottom).
- Label each tube as "Plasma."
- Immediately freeze samples
Transport Container
Plastic vial
Specimen Stability
Room temperature: 4 hours
Refrigerated: Unacceptable
Frozen (-20° C): 2 weeks
Frozen (-70° C): 6 months
Refrigerated: Unacceptable
Frozen (-20° C): 2 weeks
Frozen (-70° C): 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Short draw, clotted, heomlyzed, specimen exceeded stability.
Setup Schedule
Monday - Friday
Report Available
1 day
Reference Range
Negative
Performing Laboratory
Inova Laboratories
2832 Juniper Street
Fairfax, VA 22031
Last Updated: May 8, 2023
Last Review: N. Wolford, May 8, 2023