Culture, Stool, Salmonella/Shigella/Campylobacter/EHEC

Test Code
CXSTO


Alias/See Also
Epic: LAB223


CPT Codes
87045

Includes

Culture for Salmonella and Shigella, Immunochromatographic Rapid tests for detection of Campylobacter antigens and Shiga Toxins 1 and 2 (Enterohemorrhagic E.coli).




Preferred Specimen

Specimen Type: Stool
Collection Container: Cary Blair transport media
Specimen Volume: Stool must be filled to line on vial



 




Minimum Volume

Stool must be filled to line on Cary Blair Container or Cary Blair container instructions must be followed. Do not exceed maximum fill.




Instructions
  1. Patient should pass stool into a clean, dry, container mounted on the toilet for this purpose.
  2. Stool must not touch liquid in the toilet.
  3. Do not use toilet paper to collect sample.
  4. Sample should not be mixed with Urine.
  5. Immediately, place stool into Cary Blair transport medium. Add enough stool to reach fill line or follow included instructions. Do not exceed maximum fill.


Transport Container
Cary Blair transport media


Specimen Stability

Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: Unacceptable




Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
The laboratory accepts one stool specimen per day, per patient, for culture, additional submissions will not be processed.
Specimen received on a diaper or scraped from a diaper are unacceptable.
Rejection will occur for the following reasons: improperly labeled specimen, insufficient volume, specimen not submitted in appropriate transport container



Methodology
Standard culture methodology for detection of Salmonella and Shigella. ImmunoCard STAT EHEC for detection of Shiga Toxins 1 and 2. ImmunoCard STAT CAMPY for detection of Campylobacter antigens.


Setup Schedule
Daily


Report Available

Shiga Toxin and Campylobacter antigen final result within 2 days, Salmonella and Shigella culture results typically 3 days (but may vary depending on the necessary workup)




Limitations

Delayed transportation to the laboratory or inappropriate transportation conditions may result in decreased viability of stool pathogens. Rule out of additional bacterial pathogens requires notification of the laboratory or additional test ordering prior to sample submission. These organisms may require special growth media and separate orders and may include: Aeromonas, Vibrio, Yersinia, Plesiomonas, Staphylococcus, Pseudomonas (See clinical significance section for test codes




Reference Range
Negative for Salmonella, Shigella, Campylobacter, Shiga Toxin


Clinical Significance
Interpretation: Growth of a pathogen suggests an infectious cause of diarrhea. Diarrhea may be caused by a number of pathogens and other factors. Potential Accompanying Tests For This Source include: Yersinia-Stool Culture (CXYER), Vibrio-Stool Culture (CXVIB) and Aeromonas/Plesiomonas- Stool Culture


Performing Laboratory

Inova Laboratories

2832 Juniper Street

Fairfax, VA 22031




Last Updated: May 15, 2023
Last Review: N. Wolford, April 27, 2023


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.