Helicobacter pylori  Culture, Rapid Urease (CLO Test)

1. After removing the CLOtest rapid urease test slide from refrigeration, lift the label far enough to expose the yellow gel. For faster test results, allow the gel to reach room temperature before inserting the biopsy (usually between 7–10 minutes).
2. With a clean applicator device (e.g., toothpick etc.) push the entire sample from the forceps beneath the surface of the gel to expose as much of the specimen to the gel as possible. Make sure that the biopsy specimen is completely immersed in the gel.
3. Re-seal the pressure-sensitive label on the slide and record the patient information as well as the time the biopsy sample was inserted.
4. Submit the CLOTest to the laboratory. The CLOtest may be transported at room temperature and must reach the laboratory within 24 hours of collection. 

Room temperature: 72 hours (after insertion of biopsy into the CLOTest cartridge gel medium)

Daily

1-2 days

A false negative reaction could result from a biopsy containing an insufficient number of organisms, a sample of intestinal metaplasia, recent ingestion of antibiotics, bismuth, proton pump inhibitors, or sucralfate, formalin contamination of the sample. False positives may be caused by failure to completely insert the biopsy into the gel (and growth of contaminating organisms).