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Culture Blood, Acid Fast
Test CodeCXBLA
Alias/See Also
Epic: LAB246
Includes
Blood culture for Mycobacteria
Preferred Specimen
Specimen Type: Blood
Collection Container: BACTEC MYCOF Lytic bottle
Specimen Volume: 3-5 mL
Minimum Volume
1 mL
Specimen Stability
Bottles must reach microbiology within 12 hours of collection. Alert the laboratory if a delay has occurred.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Broken, mislabeled, and unlabeled bottles
Methodology
BACTEC blood culture system, automated continuous monitoring using fluorescent detection.
Bottles received after 12 hours will undergo manual incubation.
Setup Schedule
Daily
Report Available
Final Negative Report Available in 6 weeks.
Positive growth will be called to the patient care provider immediately for the first bottle positive per culture. Time to final positive report depends on the extent of culture workup.
Limitations
BD BACTEC Myco/F Lytic vials are incubated at 35 °C potentially precluding the recovery of mycobacteria requiring other incubation temperatures (e.g., M. marinum, M. ulcerans, M. haemophilum). Recovery of such organisms requires additional culture methods. Alert the laboratory if these organisms are suspected.
Performing Laboratory
Inova Laboratories
2832 Juniper Street
Fairfax, VA 22031
Last Updated: May 11, 2023
Last Review: N. Wolford, May 11, 2023