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Extractable Nuclear Antigen Antibodies (Add-on)
MessageImportant Note
- This test can only be ordered as an add-on if the ANA Screen was performed at Inova Laboratories. Contact IL Client Services (703-645-6175, option 3) for add-on requests.
- ENA Antibodies can be added on up to 48 hours after collection.
Test Code
ANAAB
Alias/See Also
Not orderable in Epic, only available as an add-on test.
Includes
Sjogrens SSA (Ro), Sjogrens SSB (La), Smith (Sm), RNP, Scleroderma (Scl-70), Jo-1, dsDNA, Centromere, Histone, Chromatin, Ribosomal P, RNA Polymerase III
Preferred Specimen
Specimen Type: Serum
Collection Container: Serum gel
Specimen Volume: 3 mL
Minimum Volume
1 mL
Instructions
Centrifuge and separate cells after clot formation and within 4 hours of collection.
Transport Container
Plastic vial
Specimen Stability
Room temperature: 8 hours
Refrigerated: 48 hours
Frozen: > 48 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unlabeled, mislabeled, wrong tube type, gross hemolyzed, QNS, exceeds specimen stability requirements.
Methodology
Antibodies: ELISA
Setup Schedule
Monday - Friday
Report Available
2 days
Reference Range
See laboratory report
Clinical Significance
Measurement of antinuclear antibodies (ANA) in serum is the most commonly performed screening test for patients suspected of having a systemic rheumatic disease, also referred to as connective tissue disease.(1) ANA occur in patients with a variety of autoimmune diseases, both systemic and organ-specific. They are particularly common in the systemic rheumatic diseases, which include lupus erythematosus (LE), discoid LE, drug-induced LE, mixed connective tissue disease, Sjogren syndrome, scleroderma (systemic sclerosis), CREST (calcinosis, Raynaud phenomenon, esophageal dysmotility, sclerodactyly, telangiectasia) syndrome, polymyositis/dermatomyositis, and rheumatoid arthritis.(1)
The diagnosis of a systemic rheumatic disease is based primarily on the presence of compatible clinical signs and symptoms. The results of tests for autoantibodies including ANA and specific autoantibodies are ancillary. Additional diagnostic criteria include consistent histopathology or specific radiographic findings. Although individual systemic rheumatic diseases are relatively uncommon, a great many patients present with clinical findings that are compatible with a systemic rheumatic disease and large numbers of tests for ANA are ordered to eliminate the possibility of a systemic rheumatic disease.
Mayo Clinic Laboratories, Test Catalog, (https://www.mayocliniclabs.com/test-catalog/Overview/9026)
The diagnosis of a systemic rheumatic disease is based primarily on the presence of compatible clinical signs and symptoms. The results of tests for autoantibodies including ANA and specific autoantibodies are ancillary. Additional diagnostic criteria include consistent histopathology or specific radiographic findings. Although individual systemic rheumatic diseases are relatively uncommon, a great many patients present with clinical findings that are compatible with a systemic rheumatic disease and large numbers of tests for ANA are ordered to eliminate the possibility of a systemic rheumatic disease.
Mayo Clinic Laboratories, Test Catalog, (https://www.mayocliniclabs.com/test-catalog/Overview/9026)
Performing Laboratory
Inova Laboratories
2832 Juniper Street
Fairfax, VA 22031
Last Updated: May 9, 2023
Last Review: N. Wolford, May 9, 2023