HLA-B27 Antigen

Test Code

B27HL

Alias/See Also

LAB869

CPT Codes
<p>86812</p>

Preferred Specimen

Specimen Type: Whole blood

Collection Container/Type

Preferred: Green top (sodium heparin)

Acceptable: Yellow top (ACD-A or ACD-B), or Lavender top (EDTA)

Specimen Volume: 5 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.

Minimum Volume

1 mL

Other Acceptable Specimens

Whole blood collected in: Acid citrate dextrose ACD-A or B (yellow-top), or EDTA (lavender-top) tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days

Refrigerated: 5 days

Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis • Lithium heparin (green-top) tube • Clotted • Received frozen

Methodology

Flow Cytometry (FC)

Setup Schedule

Set up: Mon-Sat; Report available: 2-4 days

Report Available

2-4 days

Reference Range

Negative

Clinical Significance

HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.

Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

Last Updated: February 23, 2012

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.