Hemoglobin, Free, Plasma

Test Code

HGBFP

CPT Codes
<p>83051</p>

Preferred Specimen

Specimen Type: Plasma, Heparin

Collection Container/Type

Preferred: Green top (Sodium heparin)

Acceptable: Green top (Lithium heparin)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

Centrifuge plasma within 1 hour of collection.
Transfer the plasma to a sterile, plastic, screw-cap vial.

Minimum Volume

0.2 mL

Other Acceptable Specimens

Lithium heparin (green-top) tube

Instructions

Centrifuge plasma within 1 hour of collection. Transfer the plasma to a sterile, plastic, screw-cap vial.

Transport Container

Plastic screw-cap vial

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable

Methodology

Colorimetry (C)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Tues-Sat; Report available: 1-2 days

Report Available

Next day

Reference Range

<8.4 mg/dL

Clinical Significance

The presence of free hemoglobin in plasma is an indication of intravascular hemolysis resulting from numerousconditions, among which include Paroxysmal Nocturnal Hemoglobinuria (PNH), Sickle-Cell Disease (SCD), Thalassemias, Hereditary Spherocytosis, Microangiopathic Hemolytic Anemias, Pyruvate Kinase Deficiency, ABO Mismatch Transfusion Reaction, Cardiopulmonary Bypass or Mechanical Heart Valve-induced Anemia.
Reference: JAMA. 2005;293(13):1653-1662

Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Last Updated: February 23, 2012

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.