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Hepatitis C Viral RNA, Genotype, LiPA
Test CodeHCVGL
CPT Codes
<p>87902</p>
Preferred Specimen
Specimen Type: Plasma EDTA
Collection Container/Type
Preferred: White top (PPT potassium EDTA)
Acceptable: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions:
- Separate serum or plasma from whole blood within 24 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature.
- Transfer the plasma to a properly identified, sterile, polypropylene screw-cap vial and ship refrigerated or frozen.
Specimen Type: Serum
Collection Container/Type
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions:
- Separate serum or plasma from whole blood within 24 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature.
- Transfer the plasma to a properly identified, sterile, polypropylene screw-cap vial and ship refrigerated or frozen.
Minimum Volume
0.6 mL
Other Acceptable Specimens
Serum
Instructions
Separate serum or plasma from whole blood within 24 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature. Transfer the plasma to a properly identified, sterile, polypropylene screw-cap vial and ship refrigerated or frozen.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 42 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unspun PPT tube • Heparinized samples
Methodology
Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Set up: Daily; Report available: 2-5 days
Report Available
2-5 days
Reference Range
See Laboratory Report
Clinical Significance
Determination of hepatitis C genotype is often required to select the most appropriate direct acting agent(s) (DAA) for the treatment of hepatitis C. Some DAA's are only effective for the treatment of hepatitis C genotype 1, whereas others may be used for additional genotypes. Refer to the package inserts of the relevant DAA's for guidance. A concurrent patient HCV viral load of >=300 IU/mL is required for this test.
Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153
Last Updated: February 23, 2012