Calcitriol 1,25-Dihydroxyvitamin D

Test Code

VD125

Alias/See Also

LAB536

CPT Codes
<p>82652</p>

Includes

Vitamin D,1,25 (OH)2,Total
Vitamin D3, 1,25 (OH)2
Vitamin D2, 1,25 (OH)2

Preferred Specimen

Specimen Type: Serum

Collection Container/Type

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Additional Information: If any sample is submitted with less than 1.1 mL and needs to be repeated, the sample will be canceled with the comment: ""TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat.""

Minimum Volume

0.5 mL

Instructions

Collect blood in a red-top tube (no gel) (preferred) or serum separator tube. Allow blood to clot (30 minutes) at room temperature. Centrifuge and separate the serum from the cells and transfer serum to a clean transport tube.

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 28 days

Refrigerated: 28 days

Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • lipemia • Icteric specimens • plasma

Methodology

Chromatography/Mass Spectrometry

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Sat; Report available: 3 days

Report Available

3 days

Reference Range

Vitamin D, 1,25 (OH)2, Total
1-9 Years	31-87 pg/mL
10-13 Years	30-83 pg/mL
14-17 Years	19-83 pg/mL
Adult	18-72 pg/mL
Vitamin D3, 1,25 (OH)2	Not available
Vitamin D2, 1,25 (OH)2	Not available

Reference ranges are established for total 1,25-dihydroxy vitamin D. Values for subcomponents D2 (derived from plant or fungal sources) and D3 (derived from human or animal sources) are provided for informational purposes only.

Clinical Significance

This test measures the bioactive form of vitamin D. It is used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure. This test is not suitable for diagnosis of vitamin D deficiency and monitoring supplementation in most patients. The 25-hydroxyvitamin D test is the recommended test for those purposes (N Engl J Med. 2007;357:266-281).

Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Last Updated: October 15, 2019

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.