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Mycoplasma pneumoniae DNA, Qualitative Real-Time PCR
Test CodeAlias/See Also
LAB261
CPT Codes
<p>87581</p>
Preferred Specimen
Submit only 1 of the following specimens:
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, sputum
Collection Container/Type: Sterile container
Submission Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions:
- Sputum: Collect in a sputum collection kit or a sterile, leak-proof plastic container.
- Bronchial Lavage: Collect in a sterile, leak-proof plastic container.
Specimen Type: Swab
Sources: Throat, nasal, or nasopharyngeal
Collection Container/Type
Preferred: VCM (green-cap)
Acceptable: M4-RT, Universal transport media (UTM), or equivalent
Specimen Volume: Swab
Collection Instructions:
- Nasopharyngeal and Throat swab in M4, VCM (UTM) Media (Use only sterile Dacron or rayon swabs.)
- Do NOT use calcium alginate swabs, as they may contain substances that inhibit PCR testing.
- Break applicator sticks off near the tip to permit tightening of the cap.
Specimen Type: Fluid
Sources: Cerebrospinal
Collection Container/Type: Sterile container
Submission Container/Tube: Sterile container
Specimen Volume: 1 mL
Minimum Volume
0.35 mL
Other Acceptable Specimens
Instructions
Sputum: Collect in a sputum collection kit or a sterile, leak-proof plastic container.
Bronchial Lavage: Collect in a sterile, leak-proof plastic container.
Transport Temperature
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Calcium alginate swabs
Methodology
Real-Time Polymerase Chain Reaction
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Report Available
1-2 days
Reference Range
Clinical Significance
The diagnosis of Mycoplasma pneumoniae infection should not rely solely upon the result of a PCR assay. A positive result should be determined in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of Mycoplasma pneumoniae DNA at detectable levels in the sample tested and does not exclude the diagnosis of disease.
Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042
Last Updated: February 23, 2012