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COVID-19 (SARS-CoV-2) and Influenza A/B and RSV
Test CodeCOIRV
Alias/See Also
Epic: LAB15676
Includes
COVID-19 (SARS-CoV-2), NAA
Influenza A and B, NAA
RSV, PCR
Influenza A and B, NAA
RSV, PCR
Preferred Specimen
Specimen Type: Nasopharyngeal (NP) swab
Collection Container: Universal transport media, viral media, or equivalent
Specimen Volume: 3 mL
Other Acceptable Specimens
Specimen Type: Nasal swab
Collection Container: Universal transport media, viral media, or equivalent
Specimen Volume: 3 mL
Collection Container: Universal transport media, viral media, or equivalent
Specimen Volume: 3 mL
Instructions
Nasopharyngeal (NP) Swab Collection
- Tilt patients head back 70 degrees
- Gently and slowly insert a minitip swab through the nostril parallel to the palate (not upwards) until resistance is encounterd or the distance is equivalent to that from the ear to the nostril of the patient, indicating contact with the nasopharynx. Swab should reach depth equal to the distance from the nostrils to outer opening of the ear.
- Gently rub and roll the swab.
- Leave swab in place for several seconds to absorb secretions.
- Slowly remove the swab while rotating it.
- Specimens can be collected from both sides using the same swab, but it is not necessary to collect specimens from both sides if the minitip is saturated with fluid from the first collection. If a deviated septum or blockage creates difficulty in obtaining the specimen from one nostril, use the same swab to obtain the specimen from the other nostril.
- Break the swab into the provided transport media for submission to the laboratory.
Anterior Nares Swab Collection
- Using a flocked or spun polyester swab, insert the swab 1/2 - 3/4 of an inch inside the nostril.
- Firmly sample the nasal membrane by rubbing with moderate pressure against as much of the wall of the anterior nares region as possible, moving the tip through a large circular path inside the nostril for 10-15 seconds.
- Repeat collection with the same swab in the second nostril.
- Break the swab into the provided transport media for submission to the laboratory
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Undefined
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimen received mislabeled/unlabeled, submitted in improper transport media or swab, leaking specimens, bloody or contain excessive mucous.
Methodology
RT-PCR
Setup Schedule
Daily
Report Available
Same day
Limitations
Negative results do not preclude SARS-CoV-2, influenza A virus, influenza B virus and/or RSV infection and should not be used as the sole basis for treatment or other patient management decisions. Negative results must be combined with clinical observations, patient history, and/or epidemiological information.
Reference Range
See laboratory report
Clinical Significance
Simultaneous qualitative detection and differentiation of SARS-CoV-2, Flu A, Flu B, and RSV viral RNA to aid in diagnosis and treatment of respiratory pathogens.
Performing Laboratory
Inova Fairfax Medical Campus Laboratory
3300 Gallows Road
Falls Church, VA 22042
Last Updated: November 1, 2023
Last Review: N. Wolford, November 1, 2023