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Group B Strep Intrapartum PCR
Test CodeGBPCR
Alias/See Also
Epic: LAB2287
Preferred Specimen
Specimen Type: Vaginal/Anal swab
Collection Container: Liquid Stuart medium (dual sterile swab)
Specimen Volume: Swab
Instructions
- Wipe away excessive amounts of secretion or discharge.
- Remove both swabs from the transport container.
- Carefully insert both swabs into the patient's vagina. Sample secretions from the mucosa of the lower one-third part of the vagina. Rotate the swabs three times to ensure uniform sample on both swabs.
- Using the same swabs, carefully insert both swabs approximately 2.5 cm beyond the anal sphincter, and gently rotate to sample anal crypts.
- Place both swabs in the transport container.
Specimen Stability
Room temperature: 24 hours
Refrigerated: 6 days
Frozen:
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Improperly labeled specimens, insufficient volume, specimens submitted in inappropriate transport containers, dried specimens or no swabs received, specimens submitted at inappropriate temperatures.
Methodology
RT-PCR
Setup Schedule
Daily
Report Available
Same day
Limitations
The Xpert GBS assay does not provide susceptibility results. Culture isolates are needed for performing susceptibility testing as recommended for penicillin-allergic women.
A negative result does not rule out the possibility of GBS colonization. False negative results may occur when the GBS concentration in the specimen is below the limit of detection (LOD).Group
A negative result does not rule out the possibility of GBS colonization. False negative results may occur when the GBS concentration in the specimen is below the limit of detection (LOD).Group
Reference Range
See laboratory report
Clinical Significance
GBS bacterial infection is associated with serious illness in newborns born to women who are colonized with GBS. Testing may provide time to administer antibiotics before delivery, if required.
Performing Laboratory
Inova Fairfax Medical Campus Laboratory
3300 Gallows Road
Falls Church, VA 22042
Last Updated: October 31, 2023
Last Review: N. Wolford, October 31, 2023