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MSH2, IHC without Interpretation
Test Code16971
CPT Codes
88342
Preferred Specimen
Formalin-fixed, paraffin-embedded tissue block submitted in an IHC specimen transport kit
Other Acceptable Specimens
5 unstained, (+) positively charged tissue slides
Instructions
Do not freeze
Transport Container
IHC Specimen Transport Kit
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Indefinitely
Refrigerated: Indefinitely
Frozen: Unacceptable
Refrigerated: Indefinitely
Frozen: Unacceptable
Methodology
Immunohistochemistry (IHC)
Setup Schedule
Set up: Mon-Fri; Report available: 2 days
Clinical Significance
Human mismatch repair protein 2 (MSH2) is involved in the initial recognition of mismatched nucleotides during the postreplication mismatch repair process. Therefore, the loss of MSH2 function leads to the accumulation of replication errors, which in turn may be responsible for the multiple mutations required for multistage carcinogenesis. Mutations in mismatch repair genes have been linked to hereditary nonpolyposis colon cancer and to sporadic cancers that exhibit microsatellite instability. MSH2 is reported to be expressed in the nuclei of cells from a variety of tissues include thyroid, heart, smooth muscle, and the germinal centers of lymphoid follicles. In ileum and colon, MSH2 expression has been reported in the crypts, the cells of which are undergoing rapid renewal. They are responsible for the continuous production of differentiated cells which migrate over 2 to 4 days before being sloughed into the lumen.
