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Anaerobic Culture with Gram Stain
Test Code465
CPT Codes
87075, 87205
Preferred Specimen
0.5 grams tissue submitted in anaerobic transport gel vial, or 0.5 mL body fluid submitted in anaerobic transport gel vial
Minimum Volume
0.5 g tissue • 0.5 mL body fluid • 1 swab
Other Acceptable Specimens
Swab submitted in Anaerobic Transport System, or in ESwab Transport System
Instructions
Abscess or deep wound, tissue, normally sterile body fluids other than blood, transtracheal aspirations, bronchoscopic specimens obtained by the double lumen technique, suprapubic urine aspirates obtained from bladder, nephrostomy tube or suprapubic catheterization, genital specimens from these sites only (cul-de-sac aspiration, culdocentesis, percutaneous transfundal aspiration, placenta, fallopian tube, intrauterine devices, surgical specimens), exudates, aspirated pus from deep wound or abscesses and tympanocentesis fluid.
Tissues and fluids are superior to a swab specimen. If swabs must be used, collect 2 anaerobic transport swabs, one for culture and one for Gram stain or submit one anaerobic transport swab with one air dried smear or one eSwab (white cap) for both culture and Gram stain. Specimens not listed as acceptable may be processed for anaerobic culture only after consultation with a Quest Microbiology Scientific Director.
Do not refrigerate or freeze.
Do not submit syringe with needle attached. If submitting syringe, remove needle, expel air and cap syringe.
Please note: Specimens should normally be obtained from a source not colonized by anaerobes. Any specimen contaminated with normal flora will not be cultured for anaerobes. A charge will be added for each identification.
Tissues and fluids are superior to a swab specimen. If swabs must be used, collect 2 anaerobic transport swabs, one for culture and one for Gram stain or submit one anaerobic transport swab with one air dried smear or one eSwab (white cap) for both culture and Gram stain. Specimens not listed as acceptable may be processed for anaerobic culture only after consultation with a Quest Microbiology Scientific Director.
Do not refrigerate or freeze.
Do not submit syringe with needle attached. If submitting syringe, remove needle, expel air and cap syringe.
Please note: Specimens should normally be obtained from a source not colonized by anaerobes. Any specimen contaminated with normal flora will not be cultured for anaerobes. A charge will be added for each identification.
Transport Temperature
Tissue or fluid: Room temperature
Swab: Refrigerated (cold packs)
Swab: Refrigerated (cold packs)
Specimen Stability
Tissue or body fluid
Room temperature: 72 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Swab
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 72 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Swab
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen • Specimens not submitted in anaerobic transport media/device • Expired transport media • Specimens collected from the following sites: throat, nasopharyngeal, sputum or bronchoscopic • Specimens not collected by double lumen technique • Feces and rectal swabs (except for clostridium difficile culture) • Urine-voided or catheter • Vaginal • Cervical • Specimens from sites contaminated with intestinal contents (colostomy sites, draining pilonidal sinus or traumatic perforation of bowel) • Superficial wounds • External ear • External eye
Methodology
Culture • Gram Stain
Setup Schedule
Set up: Daily; Report available: 7 days
Reference Range
Varies according to specimen type
